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Universal qPCR mix and efficient and fast UEIris RT(all-in-one) mix

Addtime:2020/6/10 Views:1165 Sources:US EVERBRIGHT INC.

Polymerase Chain Reaction (PCR) won the Nobel Prize in chemistry in 1993 for the character of fast ,sensitivity and specificity. It rapidly becomes the mainstream of molecular biology diagnosis,relying on its unique advantages in  Pathogens detection.   RT-PCR&qPCR are still adivancing in academic and aplication..

UE provides a series of RT-PCR & qPCR biological reagents. Recently, one tube universal SYBR Green qPCR super mix and high fast and efficiency UEIris RT all-in-one mix are introduced as new product.


Ⅰ.Universal SYBR Green qPCR Supermix(S2024)

The new product of qPCR Supermix is suitable for different instruments without adding extra ROX,meanwhile keeping high amplification efficiency and high specificity.

The experimental effect of S2024 in different instruments.(S2024 : red, A company: blue)

1.Roche LightCycler 96(No ROX)



Amplification curve
Melting curve
Ct value linear relation

2.ABI 7500(Low ROX)




Amplification curve
Melting curve
Ct value linear relation

3.ABI StepOne(High ROX)




Amplification curve
Melting curve
Ct value linear relation


UE qPCR mix are available from stock.

Product

Fast Super  EvaGreen® qPCR Master Mix(S2008)

Universal SYBR Green qPCR Supermix (S2024)

2 X SYBR Green  qPCR Master Mix (S2014)

Unique advantages

Best performance, saturated dye(super evagreen),safe, best fluorescence value

one tube suitable in diffirent instruments, special chemical modified enzyme, good stability

High quality and cost-effective, special chemical modified enzyme, good stability

Preservation of reaction mixture

6 days in room temperature

Melting operation

Melting at room temperature, no making ice, operate easily

ROX

Add corresponding concentration Rox

No adding extra ROX

Add corresponding concentration Rox

Recommended products

replace qPCR mix on the market. Please select S2013 for HRM

Replace universal SYBR Green Mix

Repalce SYBR Green Mix of chemical modification

Usilization of experience

Saturated dye super evagreen brings you different experience

One tube is universal, and still maintains excellent sensitivity, high fluorescence value and signal-to-noise ratio

Traditional mix to meet different needs of customers


Ⅱ. UEIris RT mix---high efficient,faster,premix reaction in one tube by one-step

High efficient and high fast, 12 kb-16 kb cDNA fragment can be obtained in 10 min-15 min.

◆Outstanding thermal stability.The best reaction temperature is 55 ℃, which greatly improves the reverse transcription efficiency and the tolerance to complex templates (high GC ratio or a large number of secondary structures), with better specificity and higher yield.

◆Convinient and fast,one-step RT-PCR ,sample reaction in one tube.


UE RT mix product guide

Reaction procedure / product characteristics

Remove gDNA

Reverse transcription

Enzyme inactivation

Total reaction time

The longest range of cDNA

Template range

Reaction temprature

37

55

85

-

-

-

The new product of UEIris RT mix reaction time

2 min

15 min

5 min

22min

12 kb

1 pg-5 μg

Reaction time of UEIris RT mix

2 min

10 min

10 sec

12.2min

16 kb

1 pg-1 μg


UE New product list

Catelog Number

Product name

specifications

Price(RMB)

S2024-100T

Universal SYBR Green qPCR Supermix

20μL×100T

650

S2024-500T

20μL×500T

1500

R2020-50T

UEIris RT mix with DNaseAll-in-One

20μL×50T

780

R2020-100T

20μL×100T

1280


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