1. Product Price List

2. Experimental principles

Luciferase is a general term for enzymes that can produce bioluminescence, among which the most representative are Firefly and Renilla. Luciferase catalyzes the oxidation of luciferin, and emits fluorescence simultaneously.

Firefly & Renilla Dual Luciferase Assay Kit can detect two different luciferases (Firefly, Renilla) in a single cell at one time. After internal reference processing, the influences from cell activity and transfection efficiency can be reduced. Therefore, the dual-luciferase system reduces external interferences and makes the experimental data more credible. Moreover, there is no cross interference between Firefly and Renilla in different reaction substrates.

Normalization reaction = Experimental Group (Firefly) / Control Group (Renilla)

3. Product Features

丨  Simple: all operated in one tube, save culture dishes and time.

丨  Sensitive: the lowest detection threshold is up to 10^-20mol, can be applied in the studies like weak promoters, low-expressed/down-regulated genes and gene expression in cells with low transfection efficiency, etc.

丨  Wide linear range of enzyme concentration: ≥ 8 orders of magnitude

丨  Cell lysis: strong lysis ability, suitable for almost all cell types.

4. Applications

丨  Analysis for monoclonal antibody drug evaluation

丨  Analysis of transcription factor binding sites and promoter activity

丨  Signal transduction

丨  RNA degradation,

丨  Drug screening

丨  Animal imaging in vivo. etc.

5. Competitive product comparison

Firefly luciferase test results	Renilla luciferase test results	Ratio between Firefly and Renilla test results