Parameters 

Appearance: Orange-red solid soluble in DMSO (F3005) 

CAS No.: 121714-22-5 

Molecular Formula: C51H50Cll2N2O23 

Molecular Weight: 1129.9

Storage 

Store at -20°C and protect from light. When stored as directed, product is stable for at least 12 months. 

Description 

Fluo-3, AM ester can enter into into cells via incubation. Fluo-3 AM ester itself does not bind Ca2+, but it is readily hydrolyzed to Fluo-3 by endogenous esterases once inside cells. Fluo-3 can binds to calcium ions, which can produce strong fluorescence. The maximum excitation wavelength at 506 nm and the maximum emission wavelength at 526 nm.

Notes 

1. If a serum-containing medium is used, the esterase in the serum will decompose the AM , thereby reducing the effect of Fluo-3, AM entering the cells. In addition, the medium containing phenol red will slightly increase the background value, so you should try to remove the residue of the medium before adding the working solution. 

2. There are quenching problems with fluorescent dyes. Please avoid light to slow down the fluorescence quenching. 

3. This Fluo-3, AM is easy to absorb moisture. After removing it from the refrigerator, please make sure to open it in a dry environment at room temperature. As the reagent is extremely small, centrifuge it briefly before opening to ensure that the powder falls to the bottom of the tube. 

4. Fluo-3, AM decomposes easily in contact with water. If it cannot be used up at one time, it is recommended to store the solution in small quantities.