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Furaptra(Mag-Fura-2), tetrasodium salt
Product overview:
Parameters
Appearance: Light yellow solid soluble in water (pH > 6)
λ Ex/λ Em (pH>6) = 369/511 nm (no Mg2+); 330/491 nm (high Mg2+)
Molecular Formula: C18H10N2Na4O11
Molecular Weight: 523
Storage
Store at 4°C and protect from light. When stored as directed, product is stable for at least 12 months.
Description
Mag-Fura-2 is a UV-excitable fluorescent indicator for magnesium with a Kd of 1.9 mM. Similar to Fura-2, the excitation wavelength of Mag-Fura-2 undergoes a blue shift from 369 nm to 330 nm. Mag-Fura-2 also responds to Ca2+ but with a significantly higher Kd than Fura-2 for Ca2+.An important application of Mag-Fura-2 is its use in detecting high, transient Ca2+ concentration during Ca2+ spikes. Mag-Fura-2, tetrasodium salt can be loaded into cells by microinjection or scrape loading.
Notes
1. Usually when the concentration of calcium ion is in the range of 1-100 μM or the concentration of magnesium ion is in the range of 0.1-10 mM, the fluorescence indicator effect of this product is the best.
2. Zn2+ , Cd2+ and Gd3+ can also be combined with the fluorescent indicator, which may interfere with the experiment.