Parameters 

Appearance: Light yellow solid soluble in water (pH > 6) 

λ Ex/λ Em (pH>6) = 369/511 nm (no Mg²⁺); 330/491 nm (high Mg²⁺) 

Molecular Formula: C₁₈H₁₀N₂Na₄O₁₁ 

Molecular Weight: 523

Storage 

Store at 4°C and protect from light. When stored as directed, product is stable for at least 12 months.

Description 

Mag-Fura-2 is a UV-excitable fluorescent indicator for magnesium with a Kd of 1.9 mM. Similar to Fura-2, the excitation wavelength of Mag-Fura-2 undergoes a blue shift from 369 nm to 330 nm. Mag-Fura-2 also responds to Ca²⁺ but with a significantly higher Kd than Fura-2 for Ca²⁺.An important application of Mag-Fura-2 is its use in detecting high, transient Ca²⁺ concentration during Ca²⁺ spikes. Mag-Fura-2, tetrasodium salt can be loaded into cells by microinjection or scrape loading.

Notes 

1. Usually when the concentration of calcium ion is in the range of 1-100 μM or the concentration of magnesium ion is in the range of 0.1-10 mM, the fluorescence indicator effect of this product is the best. 

2. Zn²⁺ , Cd²⁺ and Gd³⁺ can also be combined with the fluorescent indicator, which may interfere with the experiment.