Parameters 

Appearance: Light yellow solid soluble in water (PH > 6) 

CAS No.: 132319-57-4 

Molecular Formula: C₁₈H₁₀K₄N₂O₁₁ 

Molecular Weight: 587

Storage 

Store at -20°C and protect from light. When stored as directed, product is stable for at least 12 months.

Description 

Mag-Fura-2 is a UV-excitable fluorescent indicator for magnesium with a Kd of 1.9 mM. Similar to Fura-2, the excitation wavelength of Mag-Fura-2 undergoes a blue shift from 369 nm to 330 nm. Mag-Fura-2 also responds to Ca²⁺ but with a significantly higher Kd than Fura-2 for Ca²⁺. An important application of Mag-Fura-2 is its use in detecting high, transient Ca²⁺ concentration during Ca²⁺ spikes. Mag-Fura-2/tetraammonium salt can be loaded into cells by microinjection or scrape loading.

Notes 

1. Mg²⁺ indicators are sensitive to Mg²⁺ concentrations from 0.1 to 10 mM and Ca²⁺ concentrations from 1 to 100 mM.

2. Zn²⁺ , Cd²⁺ and Gd³⁺ can also bind to the fluorescent indicator, which may interfere with the experiment.