Parameters 

Appearance: Orange red solid soluble in DMF, ethanol or DMSO 

λEx/λ Em (MeOH) = 496/705 nm 

CAS No.: 90134-00-2 

Molecular Formula: C₂₈H₃₆N₂O₃S 

Molecular Weight: 481

Storage 

Store at 4°C and protect from light. When stored as directed, product is stable for at least 12 months.

Description 

ANEP dyes are molecules that fluoresce in response to electrical potential changes in their environment. These are fast-response probes that operate by means of a change in their electronic structure, and consequently their fluorescence properties, in response to a change in the surrounding electric field. Their optical response is sufficiently fast to detect transient (millisecond) potential changes in excitable cells, including single neurons, cardiac cells, and intact brains. However, the magnitude of their potential-dependent fluorescence change is often small; fast-response probes typically show a 2-10% fluorescence change per 100 mV. Furthermore, these dyes display a potential-dependent shift in their excitation spectra, thus permitting the quantitation of membrane potential using excitation ratio measurements. Di-4-ANEPPS is internalized in cells rapidly, so it is mainly used for short-term experiments.

Notes 

1. There are quenching problems with fluorescent dyes. Please avoid light to slow down the fluorescence quenching. 

2. For your safety and health, please wear lab coats and disposable gloves.