Parameters 

Appearance: Red solid soluble in DMSO 

CAS No.: 47729-63-5 

Molecular Formula: C₂₅H₂₇Cl₄IN₄ 

Molecular Weight: 652

Storage 

Store at 4°C and protect from light. When stored as directed, product is stable for at least 12 months.

Description 

JC-1 is a membrane permeable dye widely used for determining mitochondrial membrance potential with flow cytometry or fluorescent microscopy. This dye can selectively enter the mitochondria where it reversibly changes color as membrane potentials increase. This property is due to the reversible formation of JC-1 aggregates upon membrane polarization that causes shifts in emitted light from 530 nm to 590 nm when excited at 488 nm. Both colors can be detected using FITC and PE filter, respectively. JC-1 is qualitative in regards to the shift from green to red fluorescence emission, and and can be quantitatived as measured by fluorescence intensity in both filter sets. JC-1 can be used to indicate the initiation of apoptosis.

Notes 

1. If the usage amount of jc-1 is small at one time, each tube should be properly divided to avoid repeated freeze-thaw. 

2. There are quenching problems with fluorescent dyes. Please avoid light to slow down the fluorescence quenching. 

3. For your safety and health, please wear lab coats and disposable gloves.