Parameters 

Appearance: Purple solid soluble in H₂O 

Ex/Em (in MeOH): 543/- nm, (emission in MeOH is too weak to measure) 

Ex/Em (in membranes): 510/750 nm 

Molecular Formula: C₃₀H₄₅Br₂N₃ 

Molecular Weight: 607.51

Storage 

Store at 4 ℃ and protect from light. When stored as directed, product is stable for at least 12 months.

Description 

Nerve terminal probes are a series of cationic styryl fluorescent dyes used to track synaptic activity at neuromuscular junctions or synapses. These dyes usually have a lipophilic tail (two carbon chains) and a highly hydrophilic head with cations. The NerveRed probe is a dye with three double bonds.

Cationic styryl dyes function by staining synaptic vesicles. When the dye is co-incubated with cells or tissues, the aqueous part of the dye is not fluorescent, and the lipophilic tail of the dye is inserted into the cell membrane and shows strong fluorescence. After the nerve is stimulated, it will undergo endocytosis. At this time, the dye is wrapped in the vesicle, and the intensity of the fluorescent signal indicates the number of newly formed vesicles. Conversely, during exocytosis, the dye is released from the vesicle along with the neurotransmitter, resulting in a decrease in the fluorescent signal. Therefore, changes in fluorescence intensity reflect the situation of endocytosis / exocytosis or synaptic activity. The rate of fluorescence increase (binding rate) during endocytosis and the rate of fluorescence decrease (dissociation rate) during exocytosis vary depending on the type of dye. Generally, dyes with longer lipophilic tails and more double bonds have higher affinity for the membrane and therefore have higher binding rates and lower off-rates.

NerveRed C2 dye cannot be used for fixed cell staining. NM series dyes can be used for fixed cell staining.

Notes 

1. There are quenching problems with fluorescent dyes. Please avoid light to slow down the fluorescence quenching. 

2. For your safety and health, please wear lab coats and disposable gloves.