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Propidium Iodide (PI)
Product overview:
Parameters
Appearance: Orange-red solid soluble in water or DMSO
λEx/λ Em (binding DNA)=535/617 nm
λEx/λ Em (unbound DNA) = 493/636 nm
CAS No.: 25535-16-4
Molecular Formula:C27H34I2N4
Molecular Weight: 668.40
Storage
Store at 4°C and protect from light. When stored as directed, product is stable for at least 12 months.
Description
Propidium iodide (PI) is a nucleic acid fluorescent dye that does not penetrate the intact cell membrane but is capable of staining the nucleus through the cell membrane of dead cells and advanced cells of apoptosis. Therefore, PI is often used as a fluorescent probe for the detection of apoptosis or necrosis. In flow cytometry, PI is often used in combination with other dyes such as Calcein-AM, Hoechst 33258, or Hoechst 33342 to distinguish between early and late apoptotic cells as well as dead cells. In addition, PI is often used as a counterstain for multicolor fluorescent dyeing.
Notes
1. Irritating to the human body, please pay attention to proper protection.
2. For your safety and health, please wear lab coats and disposable gloves.