Parameters 

Appearance: Orange-red solid soluble in water or DMSO 

λEx/λ Em (binding DNA)=535/617 nm 

λEx/λ Em (unbound DNA) = 493/636 nm 

CAS No.: 25535-16-4 

Molecular Formula:C27H34I2N4 

Molecular Weight: 668.40

Storage 

Store at 4°C and protect from light. When stored as directed, product is stable for at least 12 months.

Description 

Propidium iodide (PI) is a nucleic acid fluorescent dye that does not penetrate the intact cell membrane but is capable of staining the nucleus through the cell membrane of dead cells and advanced cells of apoptosis. Therefore, PI is often used as a fluorescent probe for the detection of apoptosis or necrosis. In flow cytometry, PI is often used in combination with other dyes such as Calcein-AM, Hoechst 33258, or Hoechst 33342 to distinguish between early and late apoptotic cells as well as dead cells. In addition, PI is often used as a counterstain for multicolor fluorescent dyeing.

Notes 

1. Irritating to the human body, please pay attention to proper protection. 

2. For your safety and health, please wear lab coats and disposable gloves.