Parameters 

Ex/Em: 646/697 nm 

Molecular Weight: 412.49

Storage 

Store at 4 °C and protect from light. When stored as directed, product is stable for at least 12 months.

Description 

Draq 5 is an anthraquinone dye with high affinity for double-stranded DNA. It is membrane-permeable dye that can label live or fixed/dead cells. In flow cytometry, this dye can be used to distinguish nucleated and non-nucleated cells. Draq 5 can also be used to report nuclear DNA content for ploidy and cell cycle analysis because it binds DNA stoichiometrically. In fluorescent microscopy, it can be used as a nuclear counterstain. Draq 5 has been reported for use in flow cytometric analysis, microscopy, immunocytochemistry, and cell labeling.

Draq 5 can be excited by wavelengths from 488 to 647 nm. For microscopy imaging, 633 or 647 nm sources for excitation are recommended. For flow cytometry, with excitation at 488nm, this dye can be detected with filter of 685LP dichroic mirror and 710/50 band pass; with excitation at 633 nm, it can be detected with filter of 660/20 band pass. For cell cycle analysis, a higher wavelength filter is recommended, such as 735LP dichroic mirror and 780/60 band pass, to optimize the CV for the G1 and G2/M peaks. Please make sure that your instrument is capable of detecting this dye.

Because of its broad excitation and emission properties, Draq 5 is not recommended in combination with other far-red fluorochromes excited by 488 or 633 nm laser lines.

Notes 

1. There are quenching problems with fluorescent dyes. Please avoid light to slow down the fluorescence quenching. 

2. For your safety and health, please wear lab coats and disposable gloves.