Parameters

Appearance: Off-white solid soluble in DMSO
Ex/Em: 494/517 nm (pH =8)

CAS No.: 148504-34-1

Molecular Formula: C₄₆H₄₆N₂O₂₃
Molecular Weight: 995

Storage

Store at -20°C and protect from light. Expiration date marked on the outer packing.

Description

Calcein AM is a widely used green fluorescent cell marker. Calcein AM is a cell-permeant dye that can be used to determine cell viability in most eukaryotic cells. In live cells the nonfluorescent calcein AM is converted to greenfluorescent calcein, after acetoxymethyl ester hydrolysis by intracellular esterases. Calcein AM is an excellent tool for the studies of cell membrane integrity and for long term cell tracing.

Calcein-AM has low toxicity to cells and can be used in combination with PI to distinguish between live and dead cells.

Notes

1. The ester bond of Calcein AM will decompose when it encounters moisture. Please keep it frozen at -20 ℃ to prevent moisture from entering.

2. Please use the Calcein AM stock solution after dilution, and try to use it immediately.

3. There are quenching problems with fluorescent dyes. Please avoid light to slow down the fluorescence quenching.

4. For your safety and health, please wear lab coats and disposable gloves.