Parameters 

Appearance: Off-white solid soluble in 

DMSO λEx/λEm = 492/517 nm (PH=9) (after hydrolysis) 

CAS No.: 79955-27-4 

Molecular Formula: C₂₅H₁₆O₉ 

Molecular Weight: 460.396

Storage 

Store at -20°C and protect from light

Description 

5-CFDA is a live cell fluorescent tracer probe with membrane permeability and thus can be loaded into cells via incubation. Once inside cells, the non-fluorescent 5-CFDA is hydrolyzed by intracellular esterases to 5-carboxyfluorescein. These fluorescent products can only accumulate in cells with integral cell-membrane and be detected by fluorescence microscopy or flow cytometry.

Notes 

1. There are quenching problems with fluorescent dyes. Please avoid light to slow down the fluorescence quenching. 

2. For your safety and health, please wear lab coats and disposable gloves.