Parameters 

Appearance: White solid soluble in DMSO or DMF 

Ex/Em: 492/517 nm 

Molecular Formula: C₂₉H₁₇Cl₂NO₁₁ 

Molecular Weight: 626

Storage 

Store at -20℃ and protect from light. When stored as directed, product is stable for at least 12 months.

Description 

6-CDCFDA, SE is a live cell fluorescent tracer probe with membrane permeability and thus can be loaded into cells via incubation. It can be used for in vivo and in vitro experiments on cell proliferation.

6-CDCFDA, SE is a derivative of Fluorescein diacetate (FDA), which has cell membrane permeability and does not have fluorescence itself. Once inside cells, the non-fluorescent CFDA is hydrolyzed by intracellular esterases to carboxyfluorescein succinimidyl ester (CFSE). These fluorescent products can only accumulate in cells with integral cell-membrane and can emit strong green fluorescence. CFSE can spontaneously and irreversibly bind to intracellular amino groups to couple to cellular proteins. The unconjugated 6-CDCFDA, SE is returned to the cell by passive diffusion and is removed by subsequent washing steps. The fluorescence of SE-labeled cells can be stably labeled for several months, so it is very suitable for cell community analysis.

The fluorescence of 6-CDCFDA, SE-labeled cells is very uniform, which is better than other cell-tracking fluorescent probes such as PKH26. In the process of cell division and proliferation, CFSE-labeled fluorescence can be evenly distributed to the two progeny cells, the fluorescence intensity becomes half that of the parent cell, and non-dividing and dividing cells can be detected according to the difference in fluorescence intensity. Cell division once, fluorescence intensity becomes 1/2; Cell division twice, fluorescence intensity becomes 1/4; Cell division three times, fluorescence intensity becomes 1/8. 6-CDCFDA, SE can detect splits up to eight times. 6-CDCFDA, SE-labeled cells can be used for in vitro and in vivo proliferation studies, and have the function of not staining neighboring cells. 6-CDCFDA, SE can be used to detect the proliferation of lymphocytes, fibroblasts, natural killer cells, hematopoietic progenitor cells and other cells.

6-CDCFDA, SE can not only detect cell proliferation by flow cytometry, but also quantify the number of living cells with a fluorescence microplate, or use a fluorescence microscope for uniformly stained cell trace observation.

Notes 

1. 6-CDCFDA, SE reacts with amine groups. Do not use amine-containing buffers in experiments. 

2. There are quenching problems with fluorescent dyes. Please avoid light to slow down the fluorescence quenching. 

3. For your safety and health, please wear lab coats and disposable gloves.