Parameters 

Appearance: Yellow solid 

Solubility: DiO is soluble in absolute ethanol, DMSO and DMF, and the solubility in DMSO is about 10 mg / ml. When it is difficult to dissolve, you can heat or sonicate appropriately. 

Ex/Em (MeOH): 484/501 nm 

CAS No.: 34215-57-1 

Molecular Formula: C₅₃H₈₅ClN₂O₆ 

Molecular Weight: 882

Storage 

Store at -20℃ and protect from light. When stored as directed, product is stable for at least 12 months.

Description 

DiO, also called DiOC18(3), is a green fluorescent, lipophilic carbocyanine dye that is widely used as a lipophilic tracer. It is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes.

DiO is widely used as anterograde and retrograde neuronal tracers in living and fixed tissues and cells. DiI labeling does not appreciably affect cell viability.

In addition to fluorescent labeling of cell membranes, DiO can also be used to detect cell fusion and adhesion, cell migration during development or transplantation, detect lipid diffusion in cell membranes by FRAP (Fluorescence Recovery After Photobleaching), detect cytotoxicity, and label lipoproteins.

Cells can be fixed with formaldehyde either before or after staining, but not recommended for permeabilization. DiO staining is usually less intense than that of DiI, and occasionally fails completely in fixed tissues.

Notes 

1. When DiO stains fixed cell or tissue samples, the samples should be fixed with 4% paraformaldehyde in PBS. The use of other improper fixing solutions will result in a high fluorescence background. 

2. There are quenching problems with fluorescent dyes. Please avoid light to slow down the fluorescence quenching. 

3. For your safety and health, please wear lab coats and disposable gloves.

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Yixuan Tang，Xiaoyou Wang，Jie Li，Yu Nie，Guojian Liao，Yang Yu，Chong Li

ACS NANO