Parameters 

Appearance: Dark blue-green oily solid soluble in ethanol, DMF or DMSO 

Ex/Em (MeOH): 748/780 nm 

CAS No.: 100068-60-8 

Molecular Formula: C₆₃H₁₀₁IN₂ 

Molecular Weight: 1013.4

Storage 

Store at -20℃ and protect from light. When stored as directed, product is stable for at least 12 months.

Description 

DiR is a lipophilic, near-infrared fluorescent cyanine dye. The dye is useful for labeling cytoplasmic membranes. The two long 18-carbon chains insert into the cell membrane, resulting in specific and stable cell staining with no or minimal dye transfer between cells.

DiR (near infrared fluorescence) and other cell membrane fluorescent dyes such as DiI (orange fluorescence), DiO (green fluorescence), DiD (red fluorescence) are used together to provide an effective tool for multicolor imaging and flow cytometry.

Cells can be fixed with formaldehyde either before or after staining, but not recommended for permeabilization.

Notes 

1. When DiR stains fixed cell or tissue samples, the samples should be fixed with 4% paraformaldehyde in PBS. The use of other improper fixing solutions will result in a high fluorescence background. 

2. There are quenching problems with fluorescent dyes. Please avoid light to slow down the fluorescence quenching. 

3. For your safety and health, please wear lab coats and disposable gloves.

3.Overcoming the Reticuloendothelial System Barrier to Drug Delivery with a “Don’t-Eat-Us” Strategy

Yixuan Tang，Xiaoyou Wang，Jie Li，Yu Nie，Guojian Liao，Yang Yu，Chong Li

ACS NANO