Description

Agarose excluding DNase RNase and Protease is often used for size-based separation of nucleic acids in agarose gel electrophoresis applications. TAE or TBE is often used as the electrophoresis buffer. Refer to the table below for the concentration of agarose gel and the resolution of DNA electrophoresis and the ideal electrophoresis buffer. For low resolution requirements, both TAE and TBE buffer can be used. For higher resolution requirements, lower concentration gels are beneficial to large molecular weight nucleic acids and TAE should be used. while higher concentration of gel is beneficial to improve the resolution of small molecular weight nucleic acids and TBE should be used.