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Universal DNA Gel / PCR Purification Kit
Product overview:
Contents
Component |
100T |
200T |
A : Binding Buffer |
80 mL |
160 mL |
B : Wash Buffer Concentrate |
25 mL |
50 mL |
C : Elution Buffer |
6 mL |
12 mL |
D : Spin Column |
100个 |
200个 |
E : Collection Tubes |
100个 |
200个 |
Before use, please prepare wash buffer working solution (add 96-100% ethanol) |
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Required EtOH |
100mL |
200mL |
Storage
Store at room temperature. When stored as directed, product is stable for at least 12 months.
Description
US EVERBRIGHT Universal DNA Gel / PCR Purification Kit is a silica gel-based DNA extraction product. It can extract 65 bp-10 kb DNA fragments from TAE or TBE buffer. Each column can bind up to 20 μg of DNA. DNA Gel / PCR Purification Kit can effectively remove GelRed, GelGreen and other nucleic acid dyes in the gel, and the recovery rate can reach more than 80%. The purified DNA can be used in downstream experiments such as transformation, sequencing, PCR, and digestion et al..
Notes
1. The binding buffer contains irritating compounds. Wear latex gloves and glasses during operation to avoid contamination of the skin, eyes and clothes. Mixing bleach and binding buffer can produce harmful by-products. Do not mix waste from the gel extract with bleach.
2. In step 1, cutting the gel into small pieces can greatly shorten the gel melting time (linear DNA is prone to hydrolysis under prolonged exposure to high temperature conditions), thereby improving extraction. Do not expose the gel containing DNA to UV light for a long time to reduce the damage to DNA caused by UV light.