Parameters Appearance

Solid soluble in water, PBS or saline 

CAS No.: 252.23 

Molecular Weight: 61135-33-9

Storage 

Store at -20℃. When stored as directed, product is stable for at least 12 months.

Description

EdU, 5-Ethynyl-2’-deoxyuridine, is a thymine nucleoside analogue, which can replace thymine (T) in the cell proliferation stage and penetrate into the replicating DNA molecules. It can quickly detect DNA replication activity of cells through the specific reaction based on EdU and YF ® dyes, It can detect cell proliferation quickly and accurately.

This product is applicable to the proliferation detection of cells in various tissues and organs (excluding blood vessels) of mice, rats and other animal models.

Previously, the most accurate method to detect cell proliferation was BrdU method, and EdU method was a revolutionary breakthrough of BrdU method. EdU (5-ethynyl-2-deoxyuridine) is a pyrimidine analogue that can be integrated into the DNA double strand during DNA synthesis. EdU method is based on the "Click" reaction, an atomic covalent reaction of azides and alkynes catalyzed by copper.

Instructions before experiment

This product needs to be used with YF ® 488/555/594/647A Click-iT EdU Stain Kit (C6015/C6016/C6017/C6018) staining kit.

EdU is suitable for injection of various animals in vivo, with good stability and no obvious side effects for living animals. Target tissue can be prepared into paraffin or frozen tissue sections for detection; the reagent is also suitable for cell proliferation detection in vitro.