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YF®488-Annexin V and PI Apoptosis Kit
Product overview:
Storage
Store at 4℃ and protect from light. Do not freeze. Expiration date marked on the outer packing.
Parameters
YF ®488-Annexin V: Abs/Em = 490 /515 nm
PI: Abs/Em = 535 /617 nm (binding DNA)
Description
YF®488 and PI Apoptosis Kit provides a convenient method to make a distinction between apoptotic (green) and necrotic (red) cells within the same cell population by flow cytometry or fluorescence microscopy.
YF®488- Annexin V can be used to label apoptotic cells. Annexin V is a 35-36 kilodalton, Ca2+ -dependent phospholipid-binding protein with high affinity for phosphatidylserine (PS). However, in apoptotic cells, PS is translocated from the inner to the outer leaflet of the plasma membrane, where it is available for binding to fluorescently labeled Annexin V. Compared with FITC, YF®488 has higher fluorescence brightness, and is not affected by pH in the environment, and has good light stability.
Propidium iodide (PI) is a membrane impermeant DNA- binding dye which is commonly used to selectively stain dead cells in a cell population. PI stains necrotic and late apoptotic cells with compromised membrane integrity. PI can be excited by the 488, 532, or 546 nm laser lines, and emits red fluorescence.
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3.Near-infrared upconversion–activated CRISPR-Cas9 system: A remote-controlled gene editing platform
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5.MS-275联合顺铂对食管鳞状细胞癌9706细胞氧化损伤和凋亡的影响
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郑州大学生命科学学院