Contents:

Parameters 

FITC-Annexin V: Abs/Em: 494 /518 nm 

PI: Abs/Em: 535 /617 nm (with DNA)

Storage

Store at 4℃ and protect from light. Do not freeze. Expiration date marked on the outer packing.

Description

FITC-Annexin V and PI Apoptosis Kit provides a convenient method to make a distinction between apoptotic (green) and necrotic (red) cells within the same cell population by flow cytometry or fluorescence microscopy.

Fluorescent conjugates of Annexin V can be used to label apoptotic cells. Annexin V is a 35-36 kilodalton, Ca 2+ -dependent phospholipid-binding protein with high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the inner leaflet of the cytoplasmic membrane. However, in apoptotic cells, PS is translocated from the inner to the outer leaflet of the plasma membrane, where it is available for binding to fluorescently labeled Annexin V.

Propidium iodide (PI) is a membrane impermeant DNAbinding dye which is commonly used to selectively stain dead cells in a cell population. PI is excluded by live cells and early apoptotic cells, but stains necrotic and late apoptotic cells with compromised membrane integrity. PI can be excited by the 488, 532, or 546 nm laser lines, and emits red fluorescence.

1.MTP18 overexpression contributes to tumor growth and metastasis and associates with poor survival in hepatocellular carcinoma
Zhang, Yu Li, Hui Chang, Hulin Du, Lixue  Hai, Jun Geng, Xilin Yan, Xiang
cell death and disease(2018) DOI： 10.1038/s41419-018-0987-x

2.MS-275对食管鳞癌KYSE-70细胞存活、凋亡、细胞周期以及迁移的影响
刘腾飞，周建康，黄团结，王亚苹，周馨魁，张　乐，张璐玉，陈一豪，刘红涛，关方霞，马珊珊
郑州大学学报（医学版）(2018) 5 547-552

3.Histone deacetylases inhibitor MS‐275 suppresses human esophageal squamous cell carcinoma cell growth and progression via the PI3K/Akt/mTOR pathway
Shanshan Ma  Tengfei Liu  Ling Xu  Yaping Wang  Jiankang Zhou  Tuanjie Huang  Peng Li  Hongtao Liu Yanting Zhang  Xinkui Zhou  Yuanbo Cui  Xingxing Zang  Yuming Wang  Fangxia Guan
Journal of Cellular Physiology （23 May 2019）

4.SDHC-related deficiency of SDH complex activity promotes growth and metastasis of hepatocellular carcinoma via ROS/NFκB signaling
JibinLi，NingLiang，XiaoyuLong，JingZhao，JinYang，XiaohongDu，TaoYang，PengYuan，XiaojunHuang，JianshengZhang，XianliHe，JinliangXing
Cancer Letters Volume 461, 1 October 2019, Pages 44-55

5.MTFP1 overexpression promotes the growth of oral squamous cell carcinoma by inducing ROS production
Tingying Xiao，Jian Sun，Zhankui Xing，Fuqiang Xie，Lan Yang，Wenjuan Ding
CELL BIOLOGY INTERNATIONAL