Contents

Storage 

Store at 4℃ and protect from light. Do not freeze. When stored as directed, product is stable for at least 12 months.

Spectral Characteristics 

YF®647A-Annexin V: Abs/Em: 650 /665 nm 

PI: Abs/Em: 535 /617 nm (with DNA)

Description 

YF®647A-Annexin V and PI Apoptosis Kit provides a convenient method to make a distinction between apoptotic (far red) and necrotic (red) cells within the same cell population by flow cytometry or fluorescence microscopy.

Fluorescent conjugates of Annexin V can be used to label apoptotic cells. Human anticoagulant Annexin V is a 35-36 kilodalton, Ca 2+ -dependent phospholipid-binding protein with high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the inner leaflet of the cytoplasmic membrane. However, in apoptotic cells, PS is translocated from the inner to the outer leaflet of the plasma membrane, where it is available for binding to fluorescently labeled Annexin V, which can be detected by fluorescence microscopy or flow cytometry.

Propidium iodide (PI) is a membrane impermeant DNA- binding dye which is commonly used to selectively stain dead cells in a cell population. PI is excluded by live cells and early apoptotic cells, but stains necrotic and late apoptotic cells with compromised membrane integrity. PI can be excited by the 488, 532, or 546 nm laser lines, and emits red fluorescence.

    UE-Y6026