YF®488 TUNEL Apoptosis Kit (Green fluorescence)|US Everbright Inc.

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YF®488 TUNEL Apoptosis Kit (Green fluorescence)

Product No.:T6013S/T6013L

Product specification:20T/50T

Catalogue price（RMB）:1500/3000

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Product overview:

Catalog Number and Product Size:

Catalog Number	Product name	Ex/Em (nm)	Size
T6013S	YF^®488 TUNEL Apoptosis Kit (Green fluorescence)	490/515	20T
T6013L	YF^®488 TUNEL Apoptosis Kit (Green fluorescence)	490/515	50T
T6039S	YF^®555 TUNEL Apoptosis Kit (Orange red fluorescence)	555/565	20T
T6039L	YF^®555 TUNEL Apoptosis Kit (Orange red fluorescence)	555/565	50T
T6014S	YF^®594 TUNEL Apoptosis Kit (Red fluorescence)	590/617	20T
T6014L	YF^®594 TUNEL Apoptosis Kit (Red fluorescence)	590/617	50T
T6063S	YF^®640 TUNEL Apoptosis Kit (Far red fluorescence)	642/662	20T
T6063L	YF^®640 TUNEL Apoptosis Kit (Far red fluorescence)	642/662	50T
T6067S	Cy3 TUNEL Apoptosis Kit	555/565	20T
T6067L	Cy3 TUNEL Apoptosis Kit	555/565	50T

Contents:

Component	20T	50T
A. TUNEL Equilibration Buffer	2×1 mL	5 mL
B. YF^®488/555/594/640/Cy3 TUNEL Reaction Buffer	1 mL	2×1.25 mL
C. TdT Enzyme	20 μL	50 μL
D. Proteinase K (2 mg/mL)	40 μL	100 μL
E. DNase I (2 U/μL)	5 μL	13 μL
F. 10 × DNase I Buffer	100 μL	260 μL

Storage
Store at -20°C. Component B should be protected from light to avoid repeated freezing and thawing. Expiration date marked on the outer packing.

Description

When cells undergo apoptosis, some DNA endonuclease will be activated, and these endonuclease will cut the genomic DNA between nucleosomes and generate 180 bp-200 bp DNA fragment, which is represented by the specific Ladder pattern presented in agarose gel electrophoresis. Genomic DNA double-strand or single-strand breaks will produce a large number of sticky 3'-OH ends, which can be combined with YF^®/Cy-dUTP under the catalysis of deoxyribonucleotide terminal transferase (TdT), thereby passing through Fluorescence microscopy or flow cytometry can directly detect apoptotic cells. This method is called Terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL). Since normal or proliferating cells have little DNA breakage and thus no 3'-OH formation, they are rarely stained. The Tunel method can perform in situ staining of intact single apoptotic cell nuclei or apoptotic bodies, which can accurately reflect the typical biochemical and morphological characteristics of apoptosis, and can detect a very small amount of apoptotic cells. widely used in research.

This kit has a wide range of applications and can be used to detect apoptosis in frozen or paraffin sections, as well as in cultured adherent cells or suspension cells. Selectively detects apoptotic cells rather than necrotic cells or cells with DNA strand breaks caused by irradiation and drug treatment. This kit detects cell apoptosis, which takes a short time. It only needs one step of staining reaction, and it can be detected after washing.

Notes
1. Before use, please centrifuge the product to the bottom of the tube briefly, and then perform subsequent experiments.
2. When using components A and B, please wear masks and gloves, and if they touch the skin, please rinse immediately with plenty of water.
3. Fluorescent dyes all have quenching problems, please avoid light as much as possible to slow down fluorescence quenching.
4. For your safety and health, please wear a lab coat and disposable gloves.

Instructions:

UE-T6013

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YF®488 TUNEL Apoptosis Kit (Green fluorescence)

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