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YF®488 TUNEL Apoptosis Kit (Green fluorescence)
Product overview:
Catalog Number and Product Size:
Catalog Number |
Product name |
Ex/Em (nm) |
Size |
T6013S |
YF®488 TUNEL Apoptosis Kit (Green fluorescence) |
490/515 |
20T |
T6013L |
50T |
||
T6039S |
YF®555 TUNEL Apoptosis Kit (Orange red fluorescence) |
555/565 |
20T |
T6039L |
50T |
||
T6014S |
YF®594 TUNEL Apoptosis Kit (Red fluorescence) |
590/617 |
20T |
T6014L |
50T |
||
T6063S |
YF®640 TUNEL Apoptosis Kit (Far red fluorescence) |
642/662 |
20T |
T6063L |
50T |
||
T6067S |
Cy3 TUNEL Apoptosis Kit |
555/565 |
20T |
T6067L |
50T |
Contents:
Component |
20T |
50T |
A. TUNEL Equilibration Buffer
|
2×1 mL |
5 mL |
B. YF®488/555/594/640/Cy3 TUNEL Reaction Buffer |
1 mL |
2×1.25 mL |
C. TdT Enzyme |
20 μL |
50 μL |
D. Proteinase K (2 mg/mL) |
40 μL |
100 μL |
E. DNase I (2 U/μL) |
5 μL |
13 μL |
F. 10 × DNase I Buffer |
100 μL |
260 μL |
Storage
Store at -20°C. Component B
should be protected from light to avoid repeated freezing and thawing. Expiration date marked on
the outer packing.
Description
This kit has a wide range of applications and can be used to detect
apoptosis in frozen or paraffin sections, as well as in cultured adherent cells
or suspension cells. Selectively detects apoptotic cells rather than necrotic
cells or cells with DNA strand breaks caused by irradiation and drug treatment.
This kit detects cell apoptosis, which takes a short time. It only needs one
step of staining reaction, and it can be detected after washing.
Notes
1. Before use, please centrifuge the product to the
bottom of the tube briefly, and then perform subsequent experiments.
2. When using components A and B, please wear masks
and gloves, and if they touch the skin, please rinse immediately with plenty of
water.
3. Fluorescent dyes all have quenching problems,
please avoid light as much as possible to slow down fluorescence quenching.
4. For your safety and health, please wear a lab coat
and disposable gloves.