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YF®594 TUNEL Apoptosis Kit (Red fluorescence)
Product overview:
Catalog Number and Product Size:
Catalog Number |
Product name |
Ex/Em (nm) |
Size |
T6013S |
YF®488 TUNEL Apoptosis Kit (Green fluorescence) |
490/515 |
20T |
T6013L |
50T |
||
T6039S |
YF®555 TUNEL Apoptosis Kit (Orange red fluorescence) |
555/565 |
20T |
T6039L |
50T |
||
T6014S |
YF®594 TUNEL Apoptosis Kit (Red fluorescence) |
590/617 |
20T |
T6014L |
50T |
||
T6063S |
YF®640 TUNEL Apoptosis Kit (Far red fluorescence) |
642/662 |
20T |
T6063L |
50T |
||
T6067S |
Cy3 TUNEL Apoptosis Kit |
555/565 |
20T |
T6067L |
50T |
Contents:
Component |
20T |
50T |
A. TUNEL Equilibration Buffer
|
2×1 mL |
5 mL |
B. YF®488/555/594/640/Cy3 TUNEL Reaction Buffer |
1 mL |
2×1.25 mL |
C. TdT Enzyme |
20 μL |
50 μL |
D. Proteinase K (2 mg/mL) |
40 μL |
100 μL |
E. DNase I (2 U/μL) |
5 μL |
13 μL |
F. 10 × DNase I Buffer |
100 μL |
260 μL |
Storage
Store at -20°C. Component B
should be protected from light to avoid repeated freezing and thawing. Expiration date marked on
the outer packing.
Description
When cells undergo apoptosis, some DNA endonuclease will be activated, and
these endonuclease will cut the genomic DNA between nucleosomes and generate
180 bp-200 bp DNA fragment, which is represented by the specific Ladder pattern
presented in agarose gel electrophoresis. Genomic DNA double-strand or
single-strand breaks will produce a large number of sticky 3'-OH ends, which can
be combined with YF®/Cy-dUTP under the catalysis of
deoxyribonucleotide terminal transferase (TdT), thereby passing through
Fluorescence microscopy or flow cytometry can directly detect apoptotic cells.
This method is called Terminal-deoxynucleotidyl transferase mediated nick end
labeling (TUNEL). Since normal or proliferating cells have little DNA breakage
and thus no 3'-OH formation, they are rarely stained. The Tunel method can
perform in situ staining of intact single apoptotic cell nuclei or apoptotic
bodies, which can accurately reflect the typical biochemical and morphological
characteristics of apoptosis, and can detect a very small amount of apoptotic
cells. widely used in research.
This kit has a wide range of applications and can be used to detect
apoptosis in frozen or paraffin sections, as well as in cultured adherent cells
or suspension cells. Selectively detects apoptotic cells rather than necrotic
cells or cells with DNA strand breaks caused by irradiation and drug treatment.
This kit detects cell apoptosis, which takes a short time. It only needs one
step of staining reaction, and it can be detected after washing.
Notes
1. Before use, please centrifuge the product to the bottom of the tube briefly, and then perform subsequent experiments.
2. When using components A and B, please wear masks and gloves, and if they touch the skin, please rinse immediately with plenty of water.
3. Fluorescent dyes all have quenching problems, please avoid light as much as possible to slow down fluorescence quenching.
4. For your safety and health, please wear a lab coat and disposable gloves.
Citations and references:
1.OGG1-initiated base excision repair exacerbates oxidative stress-induced parthanatos
Ruoxi Wang, Chunshuang Li, Ping Qiao, Yaoyao Xue, Xu Zheng, Hongyu Chen, Xianlu Zeng,Wenguang Liu, Istvan Boldogh, Xueqing Ba
Cell Death&Disease(2018) DOI: 10.1038/s41419-018-0680-0