Parameters 

SuperView™ 488 Abs/Em: 500/530 nm (with DNA)

Storage 

Store at -20℃. Keep dry and away from light. Expiration date marked on the outer packing.

Description 

SuperView™ 488 Caspase-3 Substrate based on caspase-3/7 provides a convenient tool for profiling apoptotic cell population based on caspase-3 activity using fluorescence microscopy or flow cytometry.

Compared with other fluorescence substrates or inhibitors of caspase based on FLICA analysis, SuperView™ 488 Caspase-3 Substrate can detect activity of caspase-3/7 without inhibiting the whole cell apoptosis process.

Substrate is made of DNA fluorescence dye coupling the caspase-3/7 DEVD recognition sequence. Substrate is initially non-fluorescent and penetrates the cell membrane into the cell Cytoplasm. In apoptotic cells, caspase-3/7 cleaves Substrate and releases high affinity Harmonic DNA staining, this dye migrates to the nucleus to mark the DNA and emit.

Bright green fluorescence. Therefore, SuperView™ 488 Caspase-3 Substrate is dual-functional, which can not only detect the activity of caspase-3/7, but also visualize the nucleus morphological changes in the progress of apoptosis. SuperView™ 488 staining can be fixed in formaldehyde and compatible with subsequent immunostaining experiments.

SuperView™ 488 Caspase-3 Substrate is dissolved in DMSO or PBS. PBS form can be used for Cells sensitive to DMSO toxicity.Cells are not sensitive to DMSO,Substrate is dissolved in DMSO form can enhance dyeing effect.

Notes 

1. Cells can be counterstained with Hoechst 33342 at a final concentration of 1 μM to stain nucleus with blue fluorescence (Ex/Em: 346/460 nm) 

2. SuperView™ 488 staining is formaldehyde-fixable and not compatible with methanol fixation. 

3. Formaldehyde-fixed SuperView™ 488-stained cells can be permeabilized with 0.1% Triton X-100 for subsequent immunostaining; however, staining brightness may be diminished after permeabilization and washing.

UE-S1005