Parameter 

Ex/Em: 480/520 nm (bond with dsDNA)

Storage 

Store at 4°C and protect from light. Expiration date marked on the outer packing.

Description 

PicoGreen is an ultra sensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) in solution. Detecting and quantitating small amounts of DNA is extremely important in a wide variety of biological applications. These include standard molecular biology techniques, such as synthesizing cDNA for library production and purifying DNA fragments for subcloning, such as quantitating DNA amplification products and detecting primers. The most commonly used technique for measuring nucleic acid concentration is the determination of absorbance at 260 nm (A260). The major disadvantages of the absorbance method are the large relative contribution of nucleotides and single-stranded nucleic acids to the signal, the interference caused by contaminants commonly found in nucleic acid preparations, the inability to distinguish between DNA and RNA, and the relative insensitivity of the assay (an A260 of 0.1 corresponds to a 5 μg/mL dsDNA solution). The quantitative detection of PicoGreen is simple and convenient, becoming the standard for the detection of residual DNA in biological products.

PicoGreen only emits fluorescence when it binds to dsDNA, and the intensity of the fluorescence is proportional to the DNA concentration. PicoGreen can detect dsDNA in the range of 25 pg/mL-1000 ng/mL with a linear relationship (R²> 0.99).

Notes 

1. There are quenching problems with fluorescent dyes. Please avoid light to slow down the fluorescence quenching. 

2. To ensure the best results, PicoGreen working fluid is best prepared when using. 

3. For your safety and health, please wear lab coats and disposable gloves.

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