Parameters
Appearance: Yellow solid (D4054)
Ex/Em (with DNA ): 360/460 nm

Molecular Formula: C₁₆H₁₇Cl₂N₅
Molecular Weight: 350.25

Storage

Store at -20°C and protect from light. When stored as directed, product is stable for at least 12 months.

Description

DAPI is a blue DNA dye that is widely used as a nuclear counterstain for fluorescence microscopy, chromosome staining, and flow cytometry. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement. DAPI has a high level of photobleaching tolerance and can be used to detect yeast mitochondrial DNA, chloroplast DNA, viral DNA, microplasm DNA, and chromosomal DNA.

At lower concentrations (~1 ug/mL), DAPI is impermeant to live cells, but useful as a nuclear counterstain in fixed cells or tissue sections. At higher concentrations (~10 ug/mL), DAPI can be used to stain live cells.

Notes

1. DAPI dye is more sensitive to staining mammalian cells than staining bacteria. It is recommended to stain with a final concentration of 10 μg / mL in PBS or 150 mM NaCl for 30 min at room temperature to stain bacteria. Dead cells are usually stained more brightly than live cells.

2. For nuclear staining, the recommended working concentration of DAPI is 0.5-10 μg / mL.

3. If you need to adjust the use concentration, please choose D4054-10 mg.

4. There are quenching problems with fluorescent dyes. Please avoid light to slow down the fluorescence quenching.

5. For your safety and health, please wear lab coats and disposable gloves.

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