Contents

Storage 

Store at 4℃. When stored as directed, product is stable for at least 12 months. Store at -20℃. When stored as directed, product is stable for at least 24 months. PI needs to be protect from light.

Description 

Analysis of nucleic acids is a common application of flow cytometry. Measurement of DNA content allows the study of cell populations in various phases of the cell cycle as well as the analysis of DNA ploidy. In a given population, cells will be distributed among three major phases of cell cycle: G₀/G₁ phase (one set of paired chromosomes per cell), S phase (DNA synthesis with variable amount of DNA), and G₂/M phase(two sets of paired chromosomes per cell, prior to cell division).DNA content can be measured using fluorescent DNA stains that exhibit emission signals proportional to DNA mass. Flow cytometric analysis of these stained populations is then used to produce a frequency histogram that reveals the various phases of the cell cycle. Univariate DNA content analysis is an established assay method and is widely used for studies in oncology, cell biology, and molecular biology.

Propidium iodide (PI) Staining Solution is used for flow cyotmetric analysis of DNA content in fixed cells., PI ,a popular red-fluorescent stain, binds to DNA by intercalating between the bases with little or no sequence preference and with a stoichiometry of one dye per 4–5 base pairs of DNA. PI also binds to RNA, necessitating treatment with RNase to distinguish between RNA and DNA staining.

1.DRP1 upregulation promotes pancreatic cancer growth and metastasis through increased aerobic glycolysis

Jing Liang，Yiping Yang，Lu Bai，Feng Li，Enxiao Li

J Gastroenterol Hepatol.