Parameters

Ex/ Em: 494/517 nm (pH=8)

Storage

Store at -20℃ and protect from light. When stored as directed, product is stable for at least 6 months.

Description

Calcein, AM is a kind of fluorescent labeling reagent for living cells. It can penetrate cell membrane and enter the cell, and be cutted by esterase inside the cell. Calcein is highly negatively charged and can be trapped in the cytoplasm of normal cells, giving off bright green fluorescence. Because of its low toxicity, Calcein, AM can be used to study cell membrane integrity and long-term cell tracing experiments. In addition, it can also be used for living cell quantification. The fluorescence signal is directly proportional to the number of living cells.(Fig.1)

Fig.1 Number of HeLa cells. Cells were inoculated in 96 well plate 24 hours before detection.