• Cell Counting Kit-8(CCK-8)

    Product No.:C6005S/C6005M/C6005L/C6005XL/C6005XXL

    Product specification:100T/500T/3000T/10000T/5×10000T

    Catalogue price(RMB):300/700/2560/7000/25000

    Can replace similar products in the market

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Product overview:


Store at 4℃ and protect from light. Long-term storage can be placed at -20℃. Expiration date marked on the outer packing.


Cell Counting Kit-8, based on WST-8 (chemical name: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfob enzene)- 2H-tetrazole monosodium salt), is a rapid and highly sensitive detection kit widely used in cell proliferation and cytotoxicity.

In the presence of electron coupling reagent, WST-8 can be reduced by dehydrogenase in mitochondria to form orange formazan, which is highly water-soluble. The depth of color is directly proportional to cell proliferation and inversely proportional to cell toxicity. OD value was measured at 450 nm wavelength by enzyme labeling instrument, which indirectly reflected the number of living cells.

WST-8 is an upgrade substitute product of MTT, which has obvious advantages compared with MTT or other similar products such as XTT or MTS. First of all, formazan, which is reduced by some dehydrogenase in mitochondria, is not water-soluble and needs to be dissolved in a specific solution; while formazan, produced by WST-8, XTT and MTS, is water-soluble, so subsequent dissolution steps can be omitted. Secondly, formazan produced by WST-8 is more soluble than that produced by XTT and MTS. Thirdly, WST-8 is more stable than XTT and MTS, which makes the experimental results more stable. In addition, WST-8 has wider linear range and higher sensitivity than MTT and XTT.


If the substance to be tested is oxidable or reductive, replace fresh culture medium (remove the culture medium, wash the cell twice with culture medium, and then add new culture medium) before adding CCK to remove the drug effect. Of course, when the drug effect is relatively small, the blank absorption after adding the drug into the culture medium can be directly deducted without changing the culture medium.



Citations and references:

1.Oncogene miR-187-5p is associated with cellular proliferation, migration,invasion, apoptosis and an increased risk of recurrence in bladder cancer

ZuweiLi ,CanbinLin,LiwenZhao,LiangZhou,XiangPan,JingQuan,XiqiPeng,WeiqingLi,HangLi,JinlingXu,WeijieXu,XinGuan,YunChen

Biomedicine & Pharmacotherapy(2018)

2.MicroRNA‑572 functions as an oncogene and a potential biomarker for renal cell carcinoma prognosis 

  • Xiang Pan,
  • Zuwei Li ,
  • Liwen Zhao,
  • Jing Quan, 
  • Liang Zhou, 
  • Jinling Xu, 
  • Weiji Xu,
  • Xin Guan, 
  • Hang Li ,
  • Shangqi Yang, 
  • Yaoting Gui

Oncology Reports(2018) 3092-3101

Lin Canbin, Li Zuwei, Chen Peijie, Quan Jing , Pan Xiang,  Zhao Liwen, Zhou Liang, Lai Yulin, He Tao,Xu Weijie,Xu Jinling,Guan Xin,Li Hang, Yang Shangqi,Hu Yimin  

    Life Sciences(2018)

4.外泌体介导乳腺癌 MCF-7 细胞对多柔比星耐药性的机制

张如月; 李朵璐; 杨哲; 郭金秀; 周玉冰;





6.A dual-enzymatically cross-linked injectable gelatin hydrogel loaded with BMSC improves neurological function recovery of traumatic brain injury in rats
Minghao Yao, Feng Gao, Ru Xu,  Junni Zhang,  Yihao Chena , Fangxia Guan
Biomaterials Science

王亚苹 张振坤 李喆 刘腾飞 黄团结 周馨魁 麻建杰 关方霞 马珊珊


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