Storage 

Store at 4℃ and protect from light. Long-term storage can be placed at -20℃. Expiration date marked on the outer packing.

Description

Cell Counting Kit-8, based on WST-8 (chemical name: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfob enzene)- 2H-tetrazole monosodium salt), is a rapid and highly sensitive detection kit widely used in cell proliferation and cytotoxicity.

In the presence of electron coupling reagent, WST-8 can be reduced by dehydrogenase in mitochondria to form orange formazan, which is highly water-soluble. The depth of color is directly proportional to cell proliferation and inversely proportional to cell toxicity. OD value was measured at 450 nm wavelength by enzyme labeling instrument, which indirectly reflected the number of living cells.

WST-8 is an upgrade substitute product of MTT, which has obvious advantages compared with MTT or other similar products such as XTT or MTS. First of all, formazan, which is reduced by some dehydrogenase in mitochondria, is not water-soluble and needs to be dissolved in a specific solution; while formazan, produced by WST-8, XTT and MTS, is water-soluble, so subsequent dissolution steps can be omitted. Secondly, formazan produced by WST-8 is more soluble than that produced by XTT and MTS. Thirdly, WST-8 is more stable than XTT and MTS, which makes the experimental results more stable. In addition, WST-8 has wider linear range and higher sensitivity than MTT and XTT.

Notes 

If the substance to be tested is oxidable or reductive, replace fresh culture medium (remove the culture medium, wash the cell twice with culture medium, and then add new culture medium) before adding CCK to remove the drug effect. Of course, when the drug effect is relatively small, the blank absorption after adding the drug into the culture medium can be directly deducted without changing the culture medium.

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2.MicroRNA‑572 functions as an oncogene and a potential biomarker for renal cell carcinoma prognosis 

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• Zuwei Li ,
• Liwen Zhao,
• Jing Quan, 
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• Xin Guan, 
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Oncology Reports(2018) 3092-3101

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郑州大学生命科学学院

6.A dual-enzymatically cross-linked injectable gelatin hydrogel loaded with BMSC improves neurological function recovery of traumatic brain injury in rats
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7.重组人MG53蛋白激活Akt/GSK-3β通路降低LPS诱导的hUC-MSCs氧化损伤
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郑州大学学报（医学版）