Storage 

Store at -20℃ and protect from light. Calcein AM is easy to be hydrolyzed, which needs to be sealed and dried for storage. The diluent working solution needs to be prepared on the same day you used. Expiration date marked on the outer packing.

Parameters 

Calcein AM: Ex/Em: 494/517 nm 

PI: Ex/Em: 535/617 nm (with DNA)

Description 

Live & Dead Viability/Cytotoxicity Assay Kit for Animal Cells (Calcein AM, PI) is a kind of double fluorescent staining kit for the detection of animal cell death. The two probes in the kit can respectively measure the esterase activity and the integrity of plasma membrane to reflect the activity of cells. The kit can be used in fluorescence microscope, flow cytometer, enzyme labeling instrument and other fluorescence detection systems.

Live & Dead Viability/Cytotoxicity Assay Kit for Animal Cells (Calcein AM, PI) can be applied to most eukaryotic mammalian cells, including some tissues of adherent nuclei, but not to fungi and yeast. Compared with trypan blue, detection results of this kit is faster, safer and more sensitive.

Experimental diagram