Parameters 

Appearance: Purple solid soluble in DMSO 

Ex/Em: 579/599 nm (in MeOH) 

CAS NO.: 167095-09-2 

Molecular Formula: C₃₂H₃₂Cl₂N₂O 

Molecular Weight: 531.52

Storage 

Store at -20℃ and protect from light. When stored as directed, product is stable for at least 6 months.

Description 

MitoScene TM Red CMXRos is a red fluorescent dye that contains chloromethyl functional groups to label mitochondria. The dye is membrane permeable and becomes brightly fluorescent upon accumulation in the mitochondrial membrane. Staining is dependent on mitochondrial membrane potential, and can be used to monitor mitochondrial membrane potential in intact cells. After staining, the sample can also be fixed with an aldehyde fixing agent according to the requirements of subsequent experiments. For immunohistochemistry and in situ hybridization experiments, cells need to be permeabilized first. MitoScene TM Red CMXRos can also stain mitochondria of permeabilized cells. The dye is suitable for double labeling experiments, and its red fluorescence can be distinguished from other green fluorescent probes well.

Traditional mitochondrial fluorescent probes such as Rhodamine 123 can also be easily clustered on functional mitochondria, but once the mitochondrial membrane potential is lost, it will be washed away. Therefore, in some experiments that require cells to fix aldehydes or contain mitochondrial energy state affecting factors, their applications are greatly restricted.

Notes 

1. There are quenching problems with fluorescent dyes. Please avoid light to slow down the fluorescence quenching. 

2. For your safety and health, please wear lab coats and disposable gloves.