Storage 

Store at -20°C and protect from light. When stored as directed, product is stable for at least 6 months.

Description 

YF® SE (or NHS ester) is a type of fluorescent dye with amino reactivity produced by US Everbright. The SE groups of these dyes can react with amino groups to produce stable amide bonds. Compared with other similar dyes on the market, YF ® is a new generation of fluorescent dyes with stronger stability, better water solubility and better fluorescence intensity.

US Everbright also offers Super-n-Stain™ Antibody Labeling Kits (S6011) in small quantities, which can label 5-100 μg antibodies in 30 minutes without the need for purification steps,which is simple and convenient.

Notes 

1. When the protein labeled by this product needs to be stored for a long time, it is recommended to add 5-10 mg / mL BSA and 0.01-0.03% NaN3 to prevent protein denaturation and microbial growth. Store at 4 °C in the dark. If glycerin is added to a final concentration of 50%, it can be stored at -20 °C. Stable for more than one year. 

2. Avoid light during operation. Stir speed should be appropriate to avoid air bubbles. 

3. When installing the chromatography column, try to make the column uniform, the surface of the column is flat, and there are no bubbles or cracks. 

4. Note that when loading the sample, add the sample when the column top buffer is tangent to the gel plane, and add the eluent when the sample reaches the tangent to the gel plane. 

5. Other factors affecting labeling efficiency include: temperature, reaction time, pH, amount of fluorescent dye and protein, etc., which need to be carefully controlled. 

6. For your safety and health, please wear lab coats and disposable gloves.