Storage 

Store at 4℃ and protect from light. When stored as directed. Expiration date marked on the outer packing.

Description 

Super ECL Prime is a value-priced, entry-level horseradish peroxidase (HRP) substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need for reoptimize conditions.

Super ECL Prime provides reliability and performance equivalent to other standard ECL substrates for detection of HRP enzyme activity.Because the luminol and peroxide reagent formulations are identical to other commercially available substrate products, one can switch to Super ECL Prime without optimize probing conditions or incubation protocols.

Notes 

1. The sensitivity of luminescent liquid exposed to strong light for a long time may be slightly reduced, which can be avoided when it is moved to dark room. 

2. Long time exposure or excessive protein will deepen the background and make the change of stripe strength lose linear relationship, while insufficient exposure will blur the stripe. 

3. The luminescent liquid is extremely sensitive, it is strongly recommended that dilute 1,000 to 4,000 times for most primary antibody and 5,000 to 10,000 times for most second antibody. High antibody concentration will cause high background or no band. 

4. NaN3 can inhibit the activity of HRP. The recovery of the second antibody should avoid the use of NaN3. If necessary, it should not exceed 0.01%.

1.S-nitrosylation of the Peroxiredoxin-2 promotes S-nitrosoglutathione-mediated lung cancer cells apoptosis via AMPK-SIRT1 pathway

Yihan Zhang, Changning Sun, Guokai Xiao, Hui Shan, Luyao Tang, Yujiao Yi, Wengong Yu & Yuchao Gu

Cell Death and Disease(2019)

2.Oridonin derivatives as potential anticancer drug candidates triggering apoptosis through mitochondrial pathway in the liver cancer cells

Dongdong Luo，Yujiao Yi，Kai Peng，Tangrong Liu，Jiayu Yang，Shan Liu，Wanzhou Zhao，Xianjun Qu，Wengong Yu，Yuchao Gu，Shengbiao Wan

European Journal of Medicinal Chemistry（Volume 178, 15 September 2019, Pages 365-379）