Storage 

Store at 4℃ and protect from light. When stored as directed. Expiration date marked on the outer packing.

Description 

Super ECL Plus is a horseradish peroxidase (HRP) substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need for reoptimize conditions.

Super ECL Plus is sensitive to detect low expression or high value protein. ECL substrate can be compatible with various membranes, sealants and wide range antibody diluents, and can meet the needs of users for immunoblotting applications with excellent performance, versatility and high cost performance. Super ECL Plus has long signal duration, under optimized conditions, the substrate incubated imprinted strip can continuously output 6 to 8 hours of detectable light signal.

Notes 

1. The sensitivity of luminescent liquid exposed to strong light for a long time may be slightly reduced, which can be avoided when it is moved to dark room. 

2. Long time exposure or excessive protein will deepen the background and make the change of stripe strength lose linear relationship, while insufficient exposure will blur the stripe. 

3. The luminescent liquid is extremely sensitive, it is strongly recommended that dilute 1,000 to 5,000 times for most primary antibody and 20,000 to 100,000 times for most second antibody. High antibody concentration will cause high background or no band. 

4. NaN3 can inhibit the activity of HRP. The recovery of the second antibody should avoid the use of NaN3. If necessary, it should not exceed 0.01%.

1.CtIP promotes G2/M arrest in etoposide‐treated HCT116 cells in a p53‐independent manner

Hongyu Chen，Jin Shan，Dandan Chen，Ruoxi Wang，Wenjing Qi，Hailong Wang，Yueshuang Ke，Wenguang Liu，Xianlu Zeng

Journal of cellular physiology(2018)

2.Meningitic Escherichia coli-induced upregulation of PDGF-B and ICAM-1 aggravates blood-brain barrier disruption and neuroinflammatory response

Rui-Cheng Yang,Xin-Yi Qu,Si-Yu Xiao,Liang Li,Bo-Jie Xu,Ji-Yang Fu,Yu-Jin Lv,Nouman Amjad,Chen Tan,Kwang Sik Kim,Huan-Chun Chen andXiang-Ru Wang

Journal of Neuroinflammation（201916:101）