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Super ECL Star
Product overview:
Storage
Store at 4℃ and protect from light , It can be placed at room temperature for a short time. Expiration date marked on the outer packing.
Description
Super ECL Star is a horseradish peroxidase (HRP) substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need for reoptimize conditions.
Super ECL Star is highly sensitive, which can be used to detect very low expression or high value proteins by HRP. Super ECL Star is super sensitive luminescent liquid for detecting antibodies and associated antigens that directly or indirectly label HRP. Super ECL Star is extremely sensitive because of its unique luminescent substrate system. Super ECL Star has long signal duration, under optimized conditions, the substrate incubated imprinted strip can continuously output 8 hours of detectable light signal.
Notes
1. 1. The sensitivity of luminescent liquid exposed to strong light for a long time may be slightly reduced, which can be avoided when it is moved to dark room.
2. Long time exposure or excessive protein will deepen the background and make the change of stripe strength lose linear relationship, while insufficient exposure will blur the stripe.
3. The luminescent liquid is extremely sensitive, it is strongly recommended that dilute 5,000 to 100,000 times for most primary antibody and 100,000 to 500,000 times for most second antibody. High antibody concentration will cause high background or no band.
4. NaN3 can inhibit the activity of HRP. The recovery of the
second antibody should avoid the use of NaN3. If necessary, it should not exceed 0.01%.
Citations and references:
Lijun Yang, Jiayu Xu, Longjun Guo, Taijie Guo, Lu Zhang, Li Feng ,Hongyan Chen, Yue Wang
journal of virology (2018) DOI: 10.1128/JVI.02095-17
2.Integrin β1 promotes peripheral entry by rabies virus
Lei Shuai, Jinliang Wang, Dandan Zhao, Zhiyuan Wen, Jinying Ge, Xijun He, Xijun Wang, Zhigao Bu
Journal of virology