Contents

Note: For micro-volume reagents, please perform instantaneous centrifugation before the start of the formal experiment

Storage 

Store at -20°C. When stored as directed, product is stable for at least 2 years.

Description 

Fusion cloning is a simple, speedy and efficient DNA directed cloning technology, which can clone the inserted fragment to any position in any vector. Minerva Super Fusion Cloning Mix can efficiently and accurately fuse the DNA fragments and the linearized vectors by identifying 15-25bp homlogous sequences at the terminal of linearized vectors, and reacting them at 50℃ for 15-60 min to complete directional cloning, and the positive rate of cloning can reached more than 95%.

Notes

1) For Cloning reactions, the reagent used for the PCR reaction is recommended: 2×Taq PCR Master Mix（Green） (Catalog No. S2045). The above products can be combined with Minerva Super Fusion Cloning Kit to obtain highly reliable results. 

2) In the final examination of electrophoresis for positive results, non-toxic, non-carcinogenic and aquatic animals friendly super gelred can be used as nucleic acid gel electrophoresis reagent.

1.The R2R3-MYB Factor FhMYB5 FromFreesia hybrida Contributes to theRegulation of Anthocyanin andProanthocyanidin Biosynthesis
Yueqing Li, Xiaotong Shan, Liudi Zhou, Ruifang Gao, Song Yang, Shucai Wang, Li Wang，Xiang Gao
Frontiers in plant science(2019)
2.A functional homologue of Arabidopsis TTG1 from Freesia interacts with bHLH proteins to regulate anthocyanin and proanthocyanidin biosynthesis in both Freesia hybrida and Arabidopsis thaliana
Xiaotong Shan，Yueqing Li，Song Yang，Ruifang Gao，Liudi Zhou，Tingting Bao，Taotao Han，Shucai Wang，Xiang Gao，Li Wang
Plant Physiology and Biochemistry（Volume 141, August 2019, Pages 60-72）
3.Efficient isolation of protoplasts from freesia callus and its application in transient expression assays
Xiaotong Shan,Yueqing Li,Liudi Zhou,Linna Tong,Chao Wei,Lijun Qiu,Xiang Gao,Li Wang
Plant Cell, Tissue and Organ Culture (PCTOC)(September 2019, Volume 138, Issue 3, pp 529–541)
4.Functional Differentiation of Duplicated Flavonoid 3-O-Glycosyltransferases in the Flavonol and Anthocyanin Biosynthesis of Freesia hybrida
Xiangyu Meng, Yueqing Li1, Tongtong Zhou, Wei Sun, Xiaotong Shan, Xiang Gao,Li Wang
Frontiers in Plant Science(18 October 2019)
5.Immune responses in mice and pigs after oral vaccination with rabies virus vectored Nipah disease vaccines
LeiShuai，JinyingGe，ZhiyuanWen，JinliangWang，XijunWang，ZhigaoBu
Veterinary Microbiology Volume 241, February 2020, 108549