Storage

Store at -20℃ and protect from light. When stored as directed, product is stable for at least 3 months.

Description

Super-n-Stain™ antibody labeling kits contain everything you need to rapidly label an antibody with YF^® dyes or biotin. Simply mix your antibody with the reaction buffer and pre-measured dye provided, followed by a brief incubation. Super-n-Stain™ Dye is no longer reactive at the end of the labeling, so the conjugate is ready for staining without further purification. The antibody will be labeled with an average of 4-6 dye/label molecules per antibody molecule. Super-n-Stain™ labeling is covalent, so labeled antibodies can be used for multiplex staining without transfer of dyes/labels between antibodies.

Super-n-Stain™ labeling can tolerate sodium azide and glycine, as well as low levels of glycerol and Tris. A microcentrifuge ultrafiltration vial is provided in the kit to rapidly remove incompatible small molecule buffer components.

A modified protocol is provided for labeling antibodies with more than 4-fold excess BSA or gelatin. Select the kit size based on the amount of IgG you want to be labeled. The optimized labeling procedure is suitable for stabilizing IgG samples with excess protein or ascites. The modified labeling protocol can also be used to label samples with IgG content lower than the lowest range, and the total protein content can reach the labeling range of the kit by adding stabilizer protein. The modified procedure is not recommended for labeling the supernatant of natural antiserum or hybridoma cell lines because the antibody content of these samples is very low relative to the total protein.

When performing direct immunofluorescence with a fluorescently-labeled antibody, you may need to use a higher concentration of antibody to achieve similar staining intensity compared to indirect immunofluorescence detection using unlabeled primary plus labeled secondary antibody. In our internal testing, indirect immunofluorescence staining results in about 3-fold signal amplification compared to direct immunofluorescence staining.

Labeled secondary antibodies will still bind to primary antibodies labeled using Super-n-Stain™ kits, therefore a secondary antibody cannot be used to distinguish an unlabeled primary antibody from a Super-n-Stain™ labeled primary antibody from the same species. Super-n-Stain™ labeled antibodies can be used as a tertiary staining antibody after standard immunofluorescence staining with primary and secondary antibodies. We also provides Super-n-Stain™ labeling kit with biotin, rifavidin labeled with YF^® Dye for secondary detection or monoclonal biotin antibody labeled with YF^® Dye.