Contents

Storage 

Store at room temperature. When stored as directed, product is stable for at least 12 months

Description 

US EVERBRIGHT's plasmid extraction kit is a kind of plasmid extraction product based on silica gel column, which can obtain high quality plasmid DNA in 25 min. Centrifugal spin column uses unique new materials to efficiently and specifically adsorb plasmid DNA. It can extract <15 kb plasmid DNA from the bacterial solution. Each column can bind up to 60 μg of plasmid DNA. The extracted plasmid can be used in downstream experiments, such as transformation, sequencing, PCR and digestion. The yield of plasmid extraction is related to the purity, the type and culture conditions of the host bacteria, the degree of cell lysis, plasmid stability, and the type of antibiotics added

Notes 

1. W1 Buffer contains irritating compounds. Wear latex gloves and glasses when handling. Avoid contamination of skin, eyes and clothing. Be careful not to inhale mouth and nose. 

2. Bacterial breeding time should not exceed 16 h. 3. In step 10, make sure that the ethanol is completely removed, and allow it to stand for a few minutes at room temperature to allow it to evaporate.