Parameters 

Appearance: Red solid soluble in DMSO and water 

Ex/Em: 546/647 nm 

CAS No.: 7240-37-1 

Molecular Weight: 1270.43

Storage 

Store at -20°C and protect from light. Expiration date marked on the outer packing.

Description 

7-AAD is a fluorescent DNA binding dye that is membrane impermeant and therefore generally excluded from viable cells. The dye is excluded by live cells and early apoptotic cells, but stains necrotic and late apoptotic cells with compromised membrane integrity. Widely used in flow cytometry detection. 7-AAD has similar fluorescence characteristics as PI and can be excited by a 488nm laser. 7-AAD has a narrower emission spectrum than PI and a longer emission wavelength, so it has less interference with other detection channels. 7-AAD is the best alternative to PI in multicolor fluorescence analysis. It can be used in combination with a variety of fluorescent dyes excited by 488 nm, such as FITC, PE.

Notes 

1. There are quenching problems with fluorescent dyes. Please avoid light to slow down the fluorescence quenching. 

2. For your safety and health, please wear lab coats and disposable gloves.