Contents

Storage 

This product should be stored at -20 ℃ ; TUNEL Reaction Buffer should be stored in dark at -20℃ and avoid repeated freezing and thawing. When stored as directed, product is stable for at least 6 months.

Note: the toxic and carcinogenic ingredients such as solid cacodylate trihydrate and cobalt chloride are contained in the DAB solution and TUNEL Reaction Buffer. Please wear masks and gloves when using. If contacting the skin, please wash with plenty of water immediately. Please treat the waste liquid as toxic substance.

Description

A remarkable feature of apoptosis is the degradation of chromosome DNA, which is a common phenomenon. The degradation is very specific and regular. DNA fragments break into different lengths are of 180 bp-200 bp, showing a specific ladder like ladder pattern in agarose gel electrophoresis. The kit uses TUNEL method and uses Terminal Deoxynucleotidyl Transferase(TdT) catalyzes the incorporation of Biotin-dUTP into the 3´-OH terminal of the broken DNA of apoptotic cells. Then it was specifically combined with Streptavidin labeled with horseradish peroxidase (Streptavidin-HRP). Finally, under the catalysis of HRP, the apoptotic cells were displayed by DAB, so that the apoptotic cells could be observed and counted by ordinary optical microscope. TUNEL can selectively detect apoptotic cells rather than necrotic cells or cells with DNA strand breaks caused by irradiation and drug therapy. In this experiment, TdT enzyme catalyzes the incorporation of dUTP into the 3’-OH end of the broken DNA strand.

Notes 

1. For your safety and health, please wear lab coats and disposable gloves. 

2. Sodium azide can inhibit HRP. Do not use reagents containing sodium azide in the experiment

   UE-T6068