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One-Step Firefly Luciferase Assay Kit
Product overview:
Contents
Component |
500 T |
1000 T |
A.One-Step Firefly Luciferase Assay Buffer |
50 mL |
100 mL |
B.D-Luciferin |
10 mg |
20 mg |
Storage
Store at -80 ℃. Component A is stable for at least 3 months at -20 ℃ and 6 months at -80 ℃. Component B is stable for at
least 6 months at -20 ℃ in dark. Firefly luciferase working
solution (A + B) is stable for one month at -20 ℃ and 3 months at -80 ℃ and do not freeze and thaw repeatedly, it is
recommended to pack in small batches
Description
Reporter gene detection is an important tool in field of modern molecular biology to analyze the potential cis elements (such as promoter, enhancer and silencer) and trans acting factors in flanking region of structural genes. Firefly luciferase is widely used in the research of gene regulation, function, drug screening and so on.
Firefly luciferase is a protein and molecular weight is about 61
kD. In the presence of ATP, magnesium ion and oxygen, it can
catalyze the oxidation of luciferin to oxyluciferin. During the oxidation of luciferin, it will emit bioluminescence.