Contents

Storage
Store at 4℃ and protect from light. Do not freeze. Expiration date marked on the outer packing.
Annexin V-PE is stored in a solution of 50 mM Tris, 100 mM NaCl, 1% BSA, 0.02% NaN₃, pH 7.4.

Spectral Characteristics
Annexin V-PE: Ex/Em=488 /578 nm
RedNucleus II: Ex/Em=635/695 nm

Description
Annexin V (membrane-linked protein-V) is a Ca²⁺-dependent phospholipid-binding protein with a molecular weight of 35-36 KD that binds selectively to phosphatidylserine (PS). PS is mainly distributed on the inner side of the cell membrane, i.e., the side adjacent to the cytoplasm. In the early stages of apoptosis, different cell type exocytose phosphatidylserine to the cell surface and are exposed to the extracellular environment. At this point, the use of fluorescent protein PE-tagged Annexin V, Annexin V-PE, in combination with the outside-out PS allows flow cytometry to directly detect the outside-out PS, an important feature of apoptosis. Normal cells are not stained by Annexin V-PE, cells undergoing apoptosis or necrosis are stained by Annexin V-PE. Annexin V-PE can be used in combination with partially non-permeable nuclear dyes (7-AAD/PI) to differentiate cells in different periods of apoptosis.

RedNucleus II provided in this kit is a Far-infrared fluorescence dye, belonging to anthraquinone compounds, which cannot penetrate the intact cell membrane of living cells and early apoptotic cells.It is impermeable, but can rapidly stain nucleus/dsDNA in dead and permeable cells. Rednucleus II is an ideal substitute of propidium iodide (PI) and 7-AAD. It can be used in combination with Annexin V-PE without compensation adjustment and has better spectral characteristics: it is not excited by ultraviolet rays, nor does it interact with PE/PE. The homologues have overlapping emission, so it can be combined with FITC, PE and violet fluorescent dyes for multi-color analysisIt. When combined with Annexin V-PE, RedNucleus II is excluded from living cells and early apoptotic cells, while late apoptotic cells and dead cells are stained with Annexin V-PE and RedNucleus II at the same time.

Annexin V-PE and RedNucleus II Apoptosis Kit can be detected by flow cytometry or other fluorescence detection equipment.

UE-A6079