Storage

Store at 4℃ and protect from light. Do not freeze. Expiration date marked on the outer packing.

Spectral Characteristics

Annexin V-APC: Abs/Em: 650 /660 nm

Description

Fluorescent conjugates of Annexin V can be used to label apoptotic cells. In normal viable cells, PS is located on the inner leaflet of the cytoplasmic membrane. However, in apoptotic cells, PS is translocated from the inner to the outer leaflet of the plasma membrane, where it is available for binding to fluorescently labeled Annexin V, which can be detected by fluorescence microscopy or flow cytometry.

Annexin V-APC can be used in combination with 7-AAD/PI to detect apoptosis. 7-AAD/PI is excluded by live cells and early apoptotic cells, but stains necrotic and late apoptotic cells with compromised membrane integrity, so as to distinguish living cells, early dead cells and late dead cells.

Notes

1. Fluorescent dyes have quenching problems. Please keep away from light during storage and use to slow down fluorescence quenching.

 UE-A6080