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  • UEIris II RT-PCR System for First-Strand cDNA Synthesis( with dsDNase)

    Product No.:R2028

    Product specification:20μL×50T/20μL×100T

    Catalogue price(RMB):565/1749

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Product overview:


Component 50 T 100 T
UEIris II RT MasterMix (5X)
200 µL
2×200 µL
dsDNase (1 U/μL)
50 µL
100 µL
RNase-free water
1 mL
2×1 mL

* UEIris II RT MasterMix contains the following components: Reverse transcriptase, RNase inhibitor, dNTPs, buffer, Oligo (dT) 20 and random primers.


Store at -20°C. When stored as directed, product is stable for at least 12 months.


UEIris II RT-PCR System for First-Strand cDNA Synthesis is a simpler system for synthesizing RNA from cDNA. It contains all the reagents required for the first-strand cDNA synthesis, and only needs to add RNA template and water for reverse transcription. The synthesized first-strand cDNA can be widely used in second-strand synthesis, hybridization, PCR amplification, and qRT-PCR.

The reverse transcriptase of UEIris II master mix is a new generation of reverse transcriptase. The enzyme has a faster reaction speed and can complete the reverse transcription reaction in only 10 minutes. The reverse transcriptase also has the characteristics of higher product yield, longer cDNA fragments, and wider use of templates.

The dsDNase specifically digests double-stranded DNA, but does not digest single-stranded DNA and RNA, and is thermally sensitive. It can be rapidly and irreversibly inactivated at 55 °C to achieve the removal of genomic contamination and the reverse transcription reaction simultaneously.


1. The purity and integrity of the RNA template is an important factor affecting the reverse transcription. 

2. If the subsequent experiment is PCR, the amount of reverse transcription product should not exceed 1/10 of the volume of the PCR system. For a 20 μL PCR system, the amount of reverse transcription product should not exceed 2 μL. 

3. The mix contains Oligo (dT) 20VN and random primers. It is not only suitable for eukaryotic mRNAs containing Poly (A) structure, but also for prokaryotic RNA without Poly (A) structure, eukaryotic rRNA, and tRNAs. The master mix is not suitable for small RNA templates such as miRNA.



Citations and references:

1.The R2R3-MYB Factor FhMYB5 FromFreesia hybrida Contributes to theRegulation of Anthocyanin andProanthocyanidin Biosynthesis
Yueqing Li, Xiaotong Shan, Liudi Zhou, Ruifang Gao, Song Yang, Shucai Wang, Li Wang,Xiang Gao
Frontiers in plant science(2019)


3.棉花乙醇酸氧化酶(Glycolate oxidase)基因的克隆及表达分析
张雪 董丽君 封润霞 姚颖 刘建凤 张书玲

4.ZNF597 is a maternally expressed imprinted gene in the Holstein breed
Shukai Gu,Junliang Li,Weina Chen,Dongjie Li,Cengceng Zhang,Ruomei Li,Da Xu,Cui Zhang,Shijie Li
Theriogenology(Volume 143, February 2020, Pages 133-138)


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