Parameters 

Appearance: Dark green solid soluble in water, DMSO or DMF

Ex/Em: 647/665 nm  

Molecular Weight: 879.12   

Extinction Coefficient: 250,000   

Substitute: Alexa Fluor 657, TRITC, DyLight 640, etc.  

Storage

Store at -20°C and protect from light. When stored as directed, product is stable for at least 12 months.

Description

The cyanine dye Sulfo-Cy5-E is a far-red fluorescent labeling dye, which is highly hydrophilic and water-soluble, and contains free, unactivated monofunctional carboxylic acid. Sulfo-Cy5-E Maleimide is a thiol reaction form of Sulfo-Cy5-E dye. Maleimide reacts with thiol groups to form thioether coupling products. The reaction can be carried out in an amine at pH = 7. In a neutral pH environment, the Maleimide group does not react with histidine or arginine.

Cyanine dyes are excellent fluorescent dyes, and their molar absorption coefficients are unmatched among fluorescent dyes. Their succinimide esters are commonly used as fatty amino-labeling reagents and are widely used for the labeling of proteins, antibodies, nucleic acids, and other biological molecules And detection. By changing the length of the methine chain, the fluorescence emission wavelength can be changed. For each additional double bond, the redshift is exactly 100 nm according to Huoffman's rule.

The water-soluble cyanine dyes Cy3 and Cy5 have become universal fluorescent markers for gene chips. In addition, the absorption of Cy5, Cy5.5, and Cy7 in the near-infrared region is very low. They are long-wavelength dyes with high fluorescence intensity and good stability. Particularly suitable for in vivo imaging of small animals in place of radioactive elements.

Notes

1. When the protein labeled by this product needs to be stored for a long time, it is recommended to add 5-10 mg / mL BSA and 0.01-0.03% NaN3 to prevent protein denaturation and microbial growth.

2. Store at 4 °C in the dark. If glycerin is added to a final concentration of 50%, it can be stored at -20 °C. Stable for more than one year.