Parameters

 λEx/λEm = 500/530 nm (with DNA)

 λEx = 471 nm (without DNA)

 Color and Form: Light orange solution

Storage

Store at -20°C, protect from light

Description

AugeGreen^TM is a DNA-binding dye for real-time quantitative PCR (qPCR). The advantages of this dye make it far superior to SYBR Green I. In addition to similar spectral properties, AugeGreen^TM has three main characteristics that distinguish it from SYBR Green I.

First of all, AugeGreen^TM has much less inhibition on PCR than SYBR Green I. Therefore, the qPCR experiment using AugeGreen^TM can use fast PCR steps. At the same time, AugeGreen^TM can use higher concentration in the experiment to obtain much stronger amplified signal than SYBR Green I. Higher concentration of AugeGreen^TM also eliminates the defect of "dye redistribution", so that AugeGreen^TM can be used not only for multiple PCR, but also for high resolution dissolution curve analysis (HRM).HRM is being increasingly used in post-PCR genotyping and heterologous double-strand analysis. Because SYBR Green I can inhibit PCR, so its concentration must be very low, so SYBR Green I can not solve the problem of dye redistribution caused by low concentration, neither can it be used for multiple PCR nor HRM. At the same time, the problem of dye redistribution may also affect the reliability of the conventional melting curve, because DNA chains with low melting point may not be detected for this reason.

Secondly, AugeGreen^TM is very stable. It will not be destroyed during normal storage, operation and PCR process.

Dyes in buffer solutions can be safely stored at room temperature or in refrigerators, or can be repeatedly frozen and

thawed. On the contrary, SYBR Green I is unstable and has stronger inhibition on PCR after degradation.

Thirdly, AugeGreen^TM is safer than SYBR Green I because it does not have cell membrane permeability. Independent laboratory tests showed that AugeGreen^TM  had neither mutagenicity nor cytotoxicity. On the contrary, although SYBR Green I itself is very weak in mutagenicity, it may inhibit the repair mechanism of normal DNA in cells and enhance its mutagenicity. Considering the wide use of PCR, its safety should be paid enough attention.