Storage 

Store at 4°C and protect from light. When stored as directed, product is stable for at least 6 months.

Description 

SYBR Green I is a commonly used fluorescent dye that binds double-stranded DNA molecules by intercalating between the DNA bases. It is used in quantitative PCR because the fluorescence can be measured at the end of each amplification cycle to determine, relatively or absolutely, how much DNA has been amplified.

SYBR Green I is one such fluorescent DNA-binding dye that can be included in PCR buffer. When bound to DNA, the dye chances structurally and becomes less mobile, causing its energy to be released as fluorescence. The fluorescence increases along with the concentration of DNA.

SYBR Green I has an excitation and emission maxima of 494 nm and 521 nm, respectively.

Notes 

1. The concentration of SYBR Green I is a key factor of real-time PCR experiments. Too low will reduce the change in fluorescence signal, resulting in low-copy samples that may not be detectable, and at high concentrations it will inhibit the PCR reaction. Therefore, when using SYBR Green I dye, the concentration should be optimized according to the actual situation. The final reaction concentration is between 1× and 0.2×. 

2. Increasing the concentration of Mg 2+ can reduce the inhibitory effect of SYBR Green I on the PCR reaction. We recommend that when using SYBR Green I for real-time PCR experiments, the concentration of Mg 2+ is 0.5 to 3 mM higher than that of ordinary PCR without SYBR Green I. 

3. For your safety and health, please wear lab coats and disposable gloves.