Storage 

Store at -20℃ and protect from light. When stored as directed, product is stable for at least 6 months.

Description 

TSA (tyramide signal amplification), also known as CSA (catalyzed signal amplification), is a kind of enzyme detection method that uses HRP to carry out high-density in situ labeling of target antigens. It can not only be used for IF / IHC signal amplification, but also for ELISA, ISH and other detection methods.

TSA technology can be used to detect low abundance targets which can not be detected by traditional methods. The signal amplification technology based on tyramide can provide strong sensitivity and detect very small amount of target antigen. TSA technology greatly reduces the dosage of antibody. TSA kit can be used in combination with traditional staining methods for polychromatic imaging, two or more tyramide reactions can be performed sequentially to mark different targets on a sample, too.

Notes 

1. After 1 × Tyramide Amplification Buffer is used for the first time, it is recommended to pack it separately in small quantity and store at - 20℃ to avoid repeated freezing and thawing. 

2. Compared with fluorescent-secondary antibody, Tyramide Kit showed higher sensitivity and stronger signal. Therefore, the concentration of the primary antibody is low in this experiment, which can reduce the background fluorescence caused by non-specific binding. We suggest setting concentration gradient of the primary antibody to get the optimal one. 

3. If you need to take the background fluorescence into consideration, it is recommended to set a negative control without incubate with primary antibody. Ensure that the negative control is not cross contaminated by reagents in positive samples during incubation and washing. For tissue samples, we also recommend imaging an unstained control (without adding antibodies or tyramide) to find out the effect of tissue spontaneous fluorescence on the background. 

4. It is recommended to use 5 μg / mL HRP conjuncts, reducing the concentration may affect the signal strength and sensitivity. 

5. It is recommended to dilute YF ® / Biotin-Tyramide at 1:200. Higher concentrations may lead to too strong signal or high background. You can explore from 1:100 to 1:1000 to find the optimal concentration. 

6. After each tyramide labeling reaction, different targets on one sample can be labeled successively by HRP quenching or antibody stripping using multiple Tyramide Kits.