Storage 

Store at -20℃ and protect from light. When stored as directed, product is stable for at least 12 months.

Description 

ATP is the most important energy molecule in cells, which can be used to measure the level of cell metabolism and has a good linear relationship with the number of living cells. Therefore, number of living cells can be reflected by ATP content. This kit can detect activity of cells or number of living cells quantitatively through the luminescent reaction of fluorescein catalyzed by ATP dependent luciferase and the content of ATP in cells by chemiluminescent signal, with high sensitivity and wide linear range. The kit is compatible with a small number of sample tests and a large number of high-throughput screening tests。

The luminescent cell viability detection reagent provided by this kit has wide linear range, high sensitivity and good stability. In 96 well plates, there is a good linear relationship between 100 and 100000 cells, but the upper limit of detection number of different cells will be different. In addition, the operation is simple, the detection reagent provided in the kit is ready to use, the readings are stable, the detection speed is fast, it only takes about 10 minutes to complete the detection, no need to wash cells, no need to replace or remove the culture medium. Compared with other common methods, such as Calcein-AM, CCK-8 and so on, luminescent method is more simple and fast.

Notes 

1. The reagent contains luciferase, which will be affected by repeated freezing and thawing. It is suggested to store it at -20℃ in dark after repacking. 

2. The reagents and cell samples should be balanced to room temperature before use to avoid the effect of enzyme catalysis. 

3. When the drug content is high, it may interfere with the luciferase reaction and affect the chemiluminescence signal. It is suggested to set up a cell culture medium control well containing drugs to eliminate the interference of solvents.