Storage 

Store at -20℃ and protect from light. When stored as directed, product is stable for at least 12 months.

Description 

CFDA SE is a cell-permeable reagent that is useful in measuring and tracking cell divisions. Upon entering a live cell, the acetate groups of CFDA SE are cleaved by intracellular esterase to create the fluorescent carboxyfluorescein succinimidyl ester (CFSE) compound. CFSE reacts with free primary amines to create a stable, covalent bond and is retained in the cytosol of cells. As a cell divides, the fluorescence intensity of CFSE is successively halved with each division, which allows distinguishing each cell generation.

Notes 

1. CFDA SE are easily hydrolyzed and deteriorates rapidly in aqueous solution. Please avoid contact with water during use. In the process of labeling cells, contact with water is within limits. 

2. CFDA SE solvent will solidify and stick to the bottom, wall or cap of the centrifuge tube at 4ºC, ice bath and other lower temperatures. It can be used after 20-25ºC water bath incubation for a while until it has completely melted. 

3. The kit optimizes the CFDA SE staining system, but it is recommended that users explore the optimal working concentration and staining time according to cell type, culture conditions and application direction. 

4. There are quenching problems with fluorescent dyes. Please avoid light to slow down the fluorescence quenching. 

5. For your safety and health, please wear lab coats and disposable gloves.